Black Pearl King Oyster Mushroom Liquid Culture

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The Black Pearl Oyster or Black Pearl King Oyster is a oyster mushroom (Pleurotus ostreatus) hybrid from Japan. It is the result of a cross between a European and an Asian Pleurotus ostreatus. It grows and looks much like Pleurotus eryngii (King Oyster) in the beginning as it matures it will look more like a Pleurotus ostreatus (Pearl Oyster).

Black Pearl Kings are less finicky than Pleurotus eryngii, and are able to grow very well indoors. Grows on the same substrates as pearl or king oysters such as masters mix or hardwood sawdust (HWFP). It is a fast substrate colonizer that fruits dense, meaty mushrooms.

Fruiting Conditions:
Temperature: 50-68F
Humidity: 85-90%

Includes 10ml liquid culture syringe + sterile needle

Recommended use: Use the syringe to inoculate your own liquid culture media to expand the mycelium into many syringes worth.

Storage: Can be left at room temperature for up to 6 months, and refrigerated for longer than 1 year.

Frequently Asked Questions

Care instructions

Liquid culture can be stored at room temperature for up to 6 months and longer than a year if refrigerated. Grain spawn and substrate should be used within 1-2 weeks of being sterilized.

What is the difference between spores and liquid culture?

A liquid culture syringe is a culture medium that contains small pieces of Mycelium which have already germinated (from spores) and began growing. Often a Liquid culture syringe contains a nutritious liquid (often sterilized) such as honey liquid culture which the Mycelium can feed on and expand.

A spore syringe contains spores suspended in a water solution (often sterilized and distilled). Spores can be viewed in a similar way seeds are viewed in a plant’s life cycle. The difference with spores is that two spores that are a genetic match must meet (under the correct conditions) then germinate to begin their life cycle. A spore syringe will create a Mycelium network with many different genetic lineages inside.

How long does it take to colonize grain spawn?

Generally it takes 3-5 days for visible growth. At this point it is best to shake the grain spawn to redistribute the mycelium. After shaking, the mycelium with rebound and colonize the grain spawn fully in 1-2 weeks.

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